STEC Detection Method based on CHROMagar STEC

has shown great efficiency at the German Reference Center for E.coli

Dr. Lothar Beutn, Federal Institute for Risk Assessment, BERLIN – Germany

Dr Lothar Beutin is one of the worldwide leading scientist in the field of Shiga-Toxin-like-producing E.coli (STEC). With his team at the Federal Institute for Risk Assessment in Berlin, he has evaluated different methods for the detection of STEC in food stuff.

The results of these two years study on salad samples were presented in the Journal of Food Microbiology, issued in October, 2011. The team concluded that the optimal culture method consisted of a pre-enrichment of the sample in Brillant Broth for 6 hours at 37°C, then platng 10µl in TBX and CHROMagar STEC and, incubaton of the plates at 44°C for 18-24H.

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